Antibody Name: JIM14
Antibody Name: JIM14
Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
Catolog#:
Isotype: IgM
Antibody Type: Monoclonal
Source Animal: Rat
Detail Info for Purified Antibody:
Immunogen: Arabinogalactan protein (AGP2)
Antigen: Arabinogalactan, Arabinogalactan protein
Plant Origin: Carrot (Daucus carota)
Working Concentration: Neat or diluted 1:10
Status of Antibody: Hybridoma supernatant
Polysaccharide Cross Reactivity: JIM14.pdf
Epitope Structure for Carbonhydrate Antigen: unknown
M.W. of Protein Antigen(KDa): 80-100
Protein Cross Reactivity: Arabinogalactan proteins from diverse plants
References:
J. P. Knox, P. J. Linstead, J. Peart, C. Cooper, and K. Roberts. (1991)
Developmentally-regulated epitopes of cell surface arabinogalactan-proteins
and their relation to root tissue pattern formation. Plant Journal 1:317-326.
E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant
cell surface epitopes in exudate gums. Carbohydr.Polym.24:281-286.
E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and
J. P. Knox. (1996) Characterization of carbohydrate structural features
recognized by anti-arabinogalactan-protein monoclonal antibodies.
Glycobiology 6:131-139.
Comments:
JIM14 binds to rhamnogalacturonan I from sycamore maple and to the exudate gum,
gum ghatti. The epitope structure recognized by JIM14 is unknown.
Immunolocalization data suggest that JIM14 recognizes an epitope distinct from
JIM13 and JIM15.
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