自主生长因子GFI1抗体

适应物种 ：Human,Mouse,Rat,Dog,Pig,Horse,Rabbit,Sheep,Xenopus laevis

应用范围 ：WB,

抗体英文名 ：GFI1

规格 ：100ul

英文名称	GFI1
中文名称	自主生长因子GFI1抗体
别    名	GFI 1; GFI1; GFI-1; GFI1_HUMAN; Growth factor independence 1; growth factor independent 1; Growth factor independent protein 1; SCN2; Zinc finger protein 163; Zinc finger protein Gfi 1; Zinc finger protein Gfi-1; Zinc finger protein Gfi1; ZNF 163; ZNF163.
研究领域	肿瘤  细胞生物  生长因子和激素  转录调节因子  锌指蛋白  表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Mouse, Rat,  (predicted: Human, Dog, Pig, Horse, Rabbit, Sheep, Xenopus laevis)
产品应用	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	45kDa
细胞定位	细胞核
性    状	Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human GFI1/ZNF163:301-400/422
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
产品介绍	Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively. Function: Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription. Subunit: Interacts with U2AF1L4. Component of RCOR-GFI-KDM1A-HDAC complexes. Interacts directly with RCOR1, KDM1A and HDAC2 (By similarity). Also interacts with HDAC1. Interacts (via the zinc-finger domain) with ARIH2; the interaction prevents GFI1 ubiquitination and proteasomal degradation. Interacts with PIAS3; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Forms a complex with EHMT2 and HDAC1 to promote 'Lys-9' dimethylation of H3 (H3K9Me2) and repress expression of target genes. Interacts directly with EHMT2. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly with AJUBA (via ITS LIM domains); the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes and, occurs independently of the SNAG domain. Interacts with SPI1; the interaction inhibits SPI1 transcriptional activity and represses SPI1-regulated macrophage-specific genes required for proper granulocyte development. Interacts with RUNX1T1; the interaction represses HDAC-mediated transcriptional activity. Interacts with RELA; the interaction occurs on liposaccharide (LPS) stimulation and controls RELA DNA binding activity and regulates endotoxin-mediated TOLL-like receptor inflammatory response. Interacts (via the C-terminal zinc fingers) with ZBTB17; the interaction results in the recruitment of GFI1 to the CDKN1A/p21 and CDKN1B promoters and repression of transcription. Subcellular Location: Nucleus. Note=Colocalizes with PIAS3 and RUNX1T1 in nuclear dots. Post-translational modifications: Ubiquitinated. DISEASE: Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients. Similarity: Contains 6 C2H2-type zinc fingers. SWISS: Q99684 Gene ID: 2672 Database links: Entrez Gene: 2672 Human Entrez Gene: 14581 Mouse Entrez Gene: 24388 Rat Omim: 600871 Human SwissProt: Q99684 Human SwissProt: P70338 Mouse SwissProt: Q07120 Rat Unigene: 73172 Human Unigene: 2078 Mouse Unigene: 453139 Mouse Unigene: 10935 Ra Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.