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库存 :大量
英文名 :Spexyte™ Intracellular pH Calibration Buffer Kit
CAS号 :/
保质期 :详见说明
供应商 :齐源生物
保存条件 :详见说明
规格 :100 Tests
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电关键词:Spexyte 细胞内pH校准缓冲液试剂盒详细如下
Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes. Intracellular pH is generally 6.8 ~7.4 in the cytosol and 4.5~6.0 in the acidic organelles. Intracellular pH changes have significant physiological effects, e.g., the pH-dependent concentration of intracellular messengers such as Ca2+ and cAMP affects cellular signaling. Several recent reports showed the dysregulated pH is emerging as a hallmark of cancer cells. Spexyte™ Intracellular pH Calibration Buffer Kit provides a range of pH calibration buffers (pH 4.5~ 8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100"150 mM K+. When used in conjunction with pH indicators, such as BCFL, AM or BCECF, AM, Spexyte™ Intracellular pH Calibration Buffer Kit can create a standard curve which is used to determine the intracellular pH.
Components
Component A: pH=4.5 Buffer 1 bottle (10 mL)
Component B: pH=5.0 Buffer 1 bottle (10 mL)
Component C: pH= 5.5 Buffer 1 bottle (10 mL)
Component D: pH=6.0 Buffer 1 bottle (10 mL)
Component E: pH=6.5 Buffer 1 bottle (10 mL)
Component F: pH=7.0 Buffer 1 bottle (10 mL)
Component G: pH=7.5 Buffer 1 bottle (10 mL)
Component H: pH=8.0 Buffer 1 bottle (10 mL)
Component I: Nigericin, free acid 1 vial (2 mg)
Component J: DMSO 1 vial (300 µL)
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