产品详情
文献和实验
相关推荐
库存 :大量
英文名 :Coelenterazine hcp *CAS 123437-32-1*
CAS号 :123437-32-1
保质期 :详见说明
供应商 :齐源生物
保存条件 :详见说明
规格 :250 ug
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电关键词:钙离子荧光探针腔肠素 hcp*CAS 123437-32-1*详细如下
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下:
货号 | 英文名称 | 规格 |
AAT-102711B2 | APC Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711C0 | APC/Cy7 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711C1 | APC/Cy7 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711C2 | APC/Cy7 Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711D0 | APC/AF750 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711D1 | APC/AF750 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711D2 | APC/AF750 Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711E0 | APC/iFluor™ 700 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711E1 | APC/iFluor™ 700 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711E2 | APC/iFluor™ 700 Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711F0 | APC/iFluor™ 750 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711F1 | APC/iFluor™ 750 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711F2 | APC/iFluor™ 750 Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711G0 | FITC Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711G1 | FITC Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711H0 | TRITC Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711H1 | TRITC Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711I0 | PacBlue Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711I1 | PacBlue Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711J0 | PacOrange Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711J1 | PacOrange Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711K0 | PE Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711K1 | PE Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711K2 | PE Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711L0 | PE/Cy5 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711L1 | PE/Cy5 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711L2 | PE/Cy5 Anti-human CD27 Antibody *O323* | 500tests |
AAT-102711M0 | PE/Cy7 Anti-human CD27 Antibody *O323* | 25tests |
AAT-102711M1 | PE/Cy7 Anti-human CD27 Antibody *O323* | 100tests |
AAT-102711M2 | PE/Cy7 Anti-human CD27 Antibody *O323* | 500tests |
上海齐源生物科技有限公司
实名认证
钻石会员
入驻年限:5年