Cell Meter 细胞活性检测试剂盒（活细胞/死细胞） *绿色/红色双重荧光*价格

库存：大量

英文名：Live or Dead™ Cell Viability Assay Kit

CAS号：/

保质期：详见说明

供应商：齐源生物

保存条件：详见说明

规格：200 Tests

齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：Cell Meter 细胞活性检测试剂盒（活细胞/死细胞） *绿色/红色双重荧光*详细如下
This Live or Dead™cell viability uses two fluorogenic indicators: calcein AM for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. Calcein AM is a hydrophobic compound that easily permeates intact live cells, and becomes strongly fluorescent upon hydrolysis by esterases. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of vial cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to DNA of dead cells. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust and accurate than the other viability assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 400 assays.
Components
Component A: CytoCalcein™ Green 2 vials, lyophilized
Component B: Propidium Iodide 1 vial (10 mM, 40 µL)
Component C: DMSO 1 vial (500 µL)
Component D: Assay Buffer 1 bottle (20 mL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：

货号	英文名称	规格
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AAT-115810C0	iFluor™ 594 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810C1	iFluor™ 594 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810D0	iFluor™ 610 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810D1	iFluor™ 610 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810E0	iFluor™ 633 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810E1	iFluor™ 633 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810F0	iFluor™ 647 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810F1	iFluor™ 647 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810G0	iFluor™ 660 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810G1	iFluor™ 660 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810H0	iFluor™ 670 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810H1	iFluor™ 670 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810I0	iFluor™ 680 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810I1	iFluor™ 680 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810J0	iFluor™ 700 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810J1	iFluor™ 700 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810K0	iFluor™ 710 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810K1	iFluor™ 710 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810L0	iFluor™ 750 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810L1	iFluor™ 750 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810M0	iFluor™ 790 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810M1	iFluor™ 790 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810N0	iFluor™ 800 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810N1	iFluor™ 800 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810O0	iFluor™ 810 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810O1	iFluor™ 810 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810P0	iFluor™ 820 Anti-human CD158d Antibody mAb#33	100tests
AAT-115810P1	iFluor™ 820 Anti-human CD158d Antibody mAb#33	500tests
AAT-115810Q0	iFluor™ 840 Anti-human CD158d Antibody mAb#33	100tests