产品详情
文献和实验
相关推荐
保存条件 :4°C, sealed storage, away from moisture and light
库存 :货期:1-2天
供应商 :MedChemExpress LLC
CAS号 :150849-52-8
规格 :10 mM * 1 mL/5 mg/10 mg/25 mg/50 mg/100 mg
MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
WST-1
CAS No. : 150849-52-8
MCE 国际站:WST-1
产品活性:WST-1 是一种水溶性四唑盐。WST-1 诱导细胞内线粒体脱氢酶进行 NADH 依赖的酶切反应,释放出水溶性的甲臜产物。WST-1 可用于细胞增殖与细胞毒性检测,通过测定 450 nm 处的吸光值来反映细胞活力。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Preparation of WST-1 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of WST-1 in 1 mL of DMSO to obtain 10 mg/mL of WST-1.
1.2 Preparation of WST-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of WST-1 working solution.
Note: Please adjust the concentration of WST-1 working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of WST-1 working solution and 1-methoxy PMS (2 mM), and then incubate at room temperature for 2 h.
2.3 Use microplate reader detect 450/690 nm OD.
相关产品:MitoSOX Red | BODIPY 493/503 | Nile Red | D-Luciferin potassium | JC-1 | FITC | Propidium Iodide | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | DiR | Calcein-AM | Thiazolyl Blue | 2-NBDG | DiI | Fluo-4 AM | TMRE | Cy5.5 | Indocyanine green | Acridine Orange hydrochloride | CY5 | BODIPY 581/591 C11 | FM4-64 | CY5-SE | PKH 26 | DiD perchlorate | Diphenylterazine
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
MedChemExpress LLC
品牌商实名认证
钻石会员
入驻年限:12年