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规格 ：1支/瓶
293 (人胚肾细胞)| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C6002 | 293 (人胚肾细胞) | 1支/瓶 | 1241.00元 |
| Organism | Tissue | Morphology | Culture Properties |
| Homo sapiens (Human) | Embryonic kidney | Epithelial | Adherent |
本细胞株详细信息如下:
| General Information | |
| Cell Line Name | 293 (Human Embryonic Kidney Cells) |
| Synonyms | HEK 293; HEK-293; 293 |
| Organism | Homo sapiens (Human) |
| Tissue | Embryonic kidney |
| Cell Type | － |
| Morphology | Epithelial |
| Disease | － |
| Strain | － |
| Biosafety Level* | 2 [Cells contain Adenovirus] |
| Age at Sampling | Fetus |
| Gender | － |
| Genetics | － |
| Ethnicity | － |
| Applications | Efficacy testing |
| Category | － |
* Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
| Characteristics | |
| Karyotype | This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells. |
| Virus Susceptibility | － |
| Derivation | － |
| Clinical Data | － |
| Antigen Expression | － |
| Receptor Expression | Vitronectin, expressed |
| Oncogene | － |
| Genes Expressed | － |
| Gene expression databases | － |
| Metastasis | － |
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in nude mice |
| Comments | Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome, it is now clear that only left end sequences are present. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta－1 subunit and the vitronectin receptor alpha－v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2). |
| Culture Method | |
| Doubling Time | － |
| Methods for Passages | Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
| Medium | DMEM (high glucose)+10% FBS |
| Special Remarks | － |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | 1:6 to 1:10 weekly |
| Growth Condition | 95% air + 5% CO2, 37ºC |
| Freeze medium | DMEM (high glucose)+20% FBS+10% DMSO，也可以订购碧云天的细胞冻存液(C0210)。 |
本细胞株经过支原体检测(Mycoplasma Test)，检测结果为阴性。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| C6002 | 293 (人胚肾细胞) | 1支/瓶 |
| — | 说明书 | 1份 |
保存条件：
对于细胞培养瓶或离心管运输的活细胞，室温3-5天有效。对于干冰运输的冻存细胞，液氮保存，长期有效；-80ºC保存，2个月有效。
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北京兰博康斯科技有限公司
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入驻年限：11年
