新冠病毒S蛋白突变|英国南非巴西突变|SARS-CoV-2 S protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag (MALS verified)

保存条件 ：-20℃

保质期 ：自收到样品之日起，冻干粉-20℃可保存一年；使用载体蛋白重悬后样品-70℃保存三个月

英文名 ：SARS-CoV-2 S protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag (MALS verified)

库存 ：现货

供应商 ：ACROBiosystems

规格 ：500ug/50ug

SARS-CoV-2 S protein, His Tag (SPN-C52Hc) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. L18F/ D80A/ D215G/ R246I/ K417N/ E484K/ N501Y/ D614G/ A701V mutations were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom and SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.

Molecular Characterization

Molecular Weight:

138.0 kDa

Purity:

>90% as determined by SDS-PAGE.

Endotoxin:

Less than 1.0 EU per μg by the LAL method.

Bioactivity: