JAK2 (R683G)

免疫原 ：A synthetic peptide from the internal region of JAK2 which includes the mutation of R683G, human origin.

亚型 ：lgG

形态 ：液体

保存条件 ：-20℃

克隆性 ：单克隆

标记物 ：FITC

适应物种 ：脊椎动物

保质期 ：24个月

抗原来源 ：蛋白

目录编号 ：TD-26314

级别 ：科研级

库存 ：999

供应商 ：武汉天德

宿主 ：Mouse

应用范围 ：ELISA, WB, IHC

浓度 ：0.5 mg/ml

靶点 ：R683G

抗体英文名 ：Anti JAK2(R683G) Mouse Monoclonal Antibody

抗体名 ：JAK2(R683G)

规格 ：100 μL

JAK2(R683G)

Cat. #:  TD-26314

Size:  100 µL

Gene Symbol:  JAK2, JTK10, THCYT3

Description:   Anti-JAK2(R683G) Mouse Monoclonal Antibody

Background:    JAK2 is a non-receptor tyrosine kinase. It is a number of the Janus kinase family and has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. JAK2 orthologs have been identified in all mammals for which complete genome genome data are available. Mutations of JAK2 have been implicated in polycythemia vera, essential thrombocythemia, and myelofibrosis as well as other myeloproliferative disorders.

Immunogen:   A synthetic peptide from the internal region of JAK2 which includes the mutation of R683G, human origin.

Applications:   ELISA, WB, IHC

Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration:   0.5 mg/ml

Host Species:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Preservative:  No

Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes R683G mutant, but not wild type JAK2 of vertebrates.

Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:    Western blot analysis of recombinant JAK2(R683G) and wild type proteins. Purified His-tagged JAK2(R683G) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-JAK2(R683G) monoclonal antibody (Cat. #TD-26314).

Immunofluorescence:   Immunofluorescence of cells expressing JAK2 proteins with Anti-JAK2(R683G) antibody. HEK293T cells were transfected with pCDNA3-GFP-JAK2(R683G) plasmid, pCDNA3-GFP-JAK2 (WT) plasmid, pCDNA3-GFP-JAK2 (R683S) plasmid or pCDNA3-GFP-JAK2 (R683T) plasmid, then fixed and stained with Anti-JAK2(R683G) monoclonal antibody (Cat. #TD-26314).