ERBB2 Monoclonal Antibody

免疫原 ：A synthetic peptide from the C-terminal region of ERBB2, human origin

亚型 ：lgG

形态 ：液体

保存条件 ：-20℃

克隆性 ：单克隆

标记物 ：FITC

适应物种 ：脊椎动物

保质期 ：24个月

抗原来源 ：蛋白

目录编号 ：TD-26127

级别 ：科研级

库存 ：999

供应商 ：武汉天德

宿主 ：Mouse

应用范围 ：ELISA, WB, IHC

浓度 ：1mg/ml

靶点 ：详询

抗体英文名 ：Anti-ERBB2 mouse monoclonal antibody

抗体名 ：ERBB2 Monoclonal Antibody

规格 ：100 μL

ERBB2 Monoclonal Antibody

Cat. #:  TD26127

Gene Symbol:  ERBB2/HER2/Neu/c-Neu

Description:   Anti-ERBB2 Mouse Monoclonal Antibody

Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important criteria in clinic evaluating of the progression of breast cancer.

Immunogen:   A synthetic peptide from the C-terminal region of ERBB2, human origin

Applications:   ELISA, WB, IHC

Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:200

Concentration:    1mg/ml

Host Species:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Preservative: No

Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:   Recognizes Erb-B2 of vertebrates.

Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:     Immunohistochemistry:    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #TD-26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.