EGFR(L747-P753)S

免疫原 ：A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.

亚型 ：lgG

形态 ：液体

保存条件 ：-20℃

克隆性 ：单克隆

标记物 ：FITC

适应物种 ：脊椎动物

保质期 ：24个月

抗原来源 ：蛋白

目录编号 ：TD-26287

级别 ：科研级

库存 ：999

供应商 ：武汉天德

宿主 ：Mouse

应用范围 ： ELISA, WB, IF, IHC

浓度 ：1mg/ml

靶点 ：L747-P753

抗体英文名 ：Anti EGFR(L747-P753)S Mouse Monoclonal Antibody

抗体名 ：EGFR (L747-P753)S

规格 ：100 μL

EGFR (L747-P753)S

Cat. #:   TD-26287

Gene Symbol:  EGFR/HER1/ERBB1

Description:   Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody

Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.

Applications:   ELISA, WB, IF, IHC

Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host Species:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:

Purity:   Purified from ascites

Preservative: No

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates.

Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  
 

Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.

Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild type protein (702-987) were blotted with anti- EGFR(L747-P753)S mouse antibody (Cat. #TD-26287).

Immunofluorescence:  
 

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L747-P753)S antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with anti- EGFR(L747-P753)S monoclonal antibody (Cat. #TD-26287).