小鼠肿瘤浸润白细胞（TIL）流式表型分析

靶点 ：Tumor infiltrating leukocytes

宿主 ：Recombinant

适应物种 ：mouse

应用范围 ：Flow cytometry

浓度 ：1:50

小鼠肿瘤浸润白细胞表型分析流式抗体组合

肿瘤浸润白细胞（Tumor-infiltrating Leukocytes）表型分析是了解实体肿瘤内部免疫细胞组成及其对肿瘤发展影响的重要手段。但由于TIL含量稀少，检测信号容易被淹没在背景噪声中，给TIL分析带来极大困难。
美天旎为TIL细胞表型分析特别优化的流式抗体组合令检测更容易，且完全基于重组表达的REAfinity抗体真正实现无背景的TIL流式分析：

• 基因工程令FcγR突变，无需使用FcγR阻断也可避免非特异结合；
• 只需一种同型对照；
• 批次间高度一致，令结果可重复；

可采用如下REAfinity流式抗体组合，对小鼠肿瘤内TIL进行表型分析。
Panel-1

Specificity	Clone	Fluorochrome	Product No.
CD8β	REA793	VioBlue	130-111-638
Lag-3	REA776	VioBright™ 515	130-111-329
TIM-3	REA602	PE	130-118-563
CD4	REA604	PE-Vio 615	130-118-316
PD-1	REA802	APC	130-111-954
CD44	REA664	APC-Vio 770	130-118-695
Viobility	N/A	405/520	130-110-206

Panel-2

Specificity	Clone	Fluorochrome	Product No.
CD8b	REA793	VioBlue	130-111-638
CD4	REA604	PE-Vio 615	130-118-316
CD44	REA664	APC-Vio 770	130-118-695
CD39	REA870	APC	130-114-251
CD103	REA789	VioBright 515	130-111-609
CXCR3	REA724	PE	130-111-087
Viobility	N/A	405/520	130-110-206

以下代表性数据展示REAfinity重组抗体与传统杂交瘤抗体在小鼠TIL检测中的性能差异。

Background staining leads to gross overestimation of different immune cell subsets in the tumor microenvironment when using hybridoma-derived antibodies.
H8N8 tumor single-cell suspensions were labeled with Viobility 405/520 Fixable Dye and the indicated (table 1)
PE-labeled hybridoma-derived antibodies or REAfinity Recombinant Antibodies in the presence or absence of FcR blocking. Staining with each PE-labeled antibody was done individually. Viable single cells were gated for the analysis on the MACSQuant® Analyzer 10.



有关小鼠肿瘤研究更多信息，可访问美天旎官网肿瘤浸润白细胞应用页面
Isolation and analysis of tumor-infiltrating leukocytes (TILs)