重组人白介素-18 （rHuIL-18）

保质期 ：36个月

保存条件 ：-20℃~-70℃

英文名 ：Recombinant Human Interleukin 18

库存 ：现货

供应商 ：特宝生物

CAS号 ：无

规格 ：25μg

描述	白介素18含157个氨基酸的多肽，分子内有四个Cys , 但不形成二硫键。
来源	酵母 (Yeast)。
分子量	18.2kDa。
纯度	≥ 97%。
内毒素含量	﹤1EU/µg，鲎试剂法测定。
生物活性	促进KG-1产生IFN-γ, 间接采用IFN-γ ELISA进行测定。 测定标准如下： IL-18 80ng/mL，分泌IFN-γ ≥15IU/mL IL-18 40ng/mL，分泌IFN-γ ≥10IU/mL 产生IFN-γ的能力会因测定细胞株的状态波动。最适的浓度由各实验室的应用决定。
配方	本品为无菌冻干粉，缓冲体系为PBS，pH7.4，0.2%HSA。
复溶	建议使用0.2ml无菌水复溶。
保存	冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。 溶解以后，该细胞因子能在2-8℃的无菌条件下保存一个月，或在-20℃到-70℃的人工除霜的冰箱中保存三个月，而无明显活性损失。 避免反复冻融。

Interleukin 18 (IL-18) is a cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kD molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbial pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
仅供实验室使用。