Myeloid-Derived Suppressor Cell Isolation Kit, mouse

保存条件 ：2−8°C

英文名 ：Myeloid-Derived Suppressor Cell Isolation Kit, mouse

库存 ：大量

供应商 ：德国美天旎生物技术有限公司

概述

骨髓来源抑制性细胞(Myeloid-derived suppressor cells, MDSCs)是一群高度异质性的非成熟髓样细胞，具有单核样(monocytic- MDSCs或 M-MDSCs)和多型核样（polymorphonuclear-MDSCs或PMN-MDSCs）两大亚群，并对获得免疫及固有免疫发挥抑制功能，仅在病理条件下大量扩增。

下游应用

该试剂盒用于从小鼠脾脏、淋巴组织或实体肿瘤组织内对Gr-1^highLy-6G+和 Gr-1^dimLy-6G–髓样细胞的分离和富集。

应用举例
Human PBMCs were stained using the MDSC Detection Kit, human and analyzed by flow cytometry using the MACSQuant^® Analyzer 10. A) To exclude red blood cells and identify leukocytes, a first gate on CD45⁺ cells was set (region R1). Next, to eliminate doublets, a gate on single cells in Forward scatter-A (A=area) versus Forward scatter-H (H=height) was set (region R2). These cells were further distinguished from debris via Forward scatter-A and Side scatter-A (region R3). Afterwards, a gate on viable cells (7-AAD^– cells) was set (region R4). B) Cells from region R4 were further separated into 3 subsets: side scatter^high (SSC^high) cells, which correspond to low density PMN-MDSCs (region R5), CD14⁺cells (region R6) and SSC^lowCD14^– cells (region R7). Low density PMN-MDSCs contained in region R5 were further separated into 4 subsets based on the expression of CD16 and CD11b (gates R8 to R11). C) Cells stained with MDSC Control Cocktail from R6 were displayed with CD14 versus REA Control-FITC, and a region enclosing >99.5% of cells was set (region R12). This region was transferred to the cells labeled with MDSC Staining Cocktail, depicting M-MDSCs as CD14⁺HLA-DR^–. D) Cells stained with MDSC Control Cocktail from region R7 were displayed with REA Control-FITC versus REA-Control-PE, and a region above the background staining, containing ^–CD33^int.

相关数据查看
https://www.miltenyibiotec.com/DE-en/products/mdsc-detection-kit-human.html#130-126-233