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应用范围 ：ELISA;Immunoprecipitation
宿主 ：Rat
抗原来源 ：0
库存 ：大量
适应物种 ：Human
是否单克隆 ：1
规格 ：100 μg
Product Images
Western Blot Analysis:
A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-TUBULIN (1:500 dilution of ...
Western Blot Analysis:
Representative image from a previous lot.
A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (La...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IH, IP, RIA, WB | Rat | Purified | Monoclonal Antibody |
Description:
Anti-Tubulin, clone YL1/2
Background Information:
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
Clone:
YL1/2
Specificity:
MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Key Applications:
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Radioimmunoassay
- Western Blotting
Application Notes:
Immunohistochemistry:
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
1:50-1:100 dilutoin from a previous lot was used on frozen sections.
ELISA:
1:100-1:1,000 dilution from a previousl lot was used.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Radioimmunoassy:
A previous lot of this antibody was used on Radioimmuoassy.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, mouse, and rat.
Isotype:
IgG2a
Immunogen:
Yeast tubulin
Epitope:
The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Molecular Weight:
55 kDa
Control:
All tissue
Presentation:
Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Quality Assurance:
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
Antibody Category:
Cell Structure
Antibody Sub-Category:
Cytoskeleton
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Rat
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Yeast tubulin 详细描述见链接：http://www.millipore.com/catalogue/item/MAB1864
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