产品详情
文献和实验
相关推荐
应用范围 ：Flow Cytometry;Immunoprecipitation
宿主 ：Mouse
抗原来源 ：0
库存 ：大量
适应物种 ：Rat
是否单克隆 ：1
规格 ：100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IC, IH, IP, RIA, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Macrophages/Monocytes, clone ED-1
Replaces:
CBL1511
Background Information:
Monocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
Clone:
ED-1
Specificity:
Recognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Radioimmunoassay
- Western Blotting
Application Notes:
Immunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Rat
Isotype:
IgG1
Immunogen:
Rat spleen cells
Molecular Weight:
90-100 kD
Control:
Macrophages, monocytes, lymphoid organs
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Antibody Category:
Inflammation & Immunology
Antibody Sub-Category:
Immunoglobulins & Immunology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Rat spleen cells 详细描述见链接：http://www.millipore.com/catalogue/item/MAB1435
默克生命科学
品牌商实名认证
金牌会员
入驻年限：9年
