Retinal Cell Line (R28) Cytospin大鼠视网膜细胞系(R28)涂片

CAS号 ：咨询销售

细胞类型 ：Immortalized Cell Lines

物种来源 ：Rat

免疫类型 ：无

细胞形态 ：咨询销售

运输方式 ：Dry Ice

年限 ：5-71年

生长状态 ：贴壁生长

规格 ：1 vial

Each Retinal Cell Line (R28) Cytospin contains approximately 500-1,000 cells fixed to a microscope slide. These convenient cytospins can immediately be used for experiments instead of taking the time to grow the actual R28 cells (e.g. to quickly and easily check and see if the cells have a particular gene of interest). The cytospins are compatible with standard immunocytochemistry techniques or in situ hybridization.The R28 cells were developed from E1A-NR.3 parental cell line through three rounds of limiting dilution and were therefore derived from a single cell. Despite their clonal origin, these cells display both glial and neuronal cell markers indicative of a retinal precursor cell. The parental line E1A-NR.3 was established by immortalization of postnatal day 6 rat neuroretinal tissue using the psi2 replication incompetent retroviral vector. As a result, these cells are already resistant to geneticin/G418 and would require an alternative selection marker for transfection studies. These cells were designed not to form tumors in animals.每个视网膜细胞系(R28)细胞涂片包含大约500- 1000个细胞固定在显微镜载玻片上。这些方便的细胞固定器可以立即用于实验，而不是花时间培养实际的R28细胞(例如，快速和容易地检查和查看细胞是否有一个特定的基因感兴趣)。这些胞质酶与标准的免疫细胞化学技术或原位杂交技术兼容。R28细胞来源于E1A-NR。3亲本细胞系经过三轮限制稀释，因此从单个细胞中提取。尽管它们的起源是克隆的，这些细胞同时显示胶质细胞和神经元细胞标记，指示视网膜前体细胞。母系E1A-NR。采用psi2复制不合格逆转录病毒载体对出生后6天大鼠神经视网膜组织进行永生化。因此，这些细胞已经对geneticin/G418具有抗性，需要另一种选择标记来进行转染研究。这些细胞被设计成不会在动物体内形成肿瘤。