Anti-Phospho-PRKCA(Thr638) Polyclonal Antibody

免疫原 ：KLH conjugated synthesised phosphopeptide derived from human PKC alpha around the phosphorylation site of Thr638

亚型 ：IgG

形态 ：冻干粉

保存条件 ：Store at -20°C. Avoid freeze / thaw cycles.

克隆性 ：单克隆

标记物 ：Cytoplasm Nucleus Cell membrane

适应物种 ：Human Mouse Rat Chicken Dog Cow Horse

保质期 ：现询客服

抗原来源 ：KLH conjugated synthesised phosphopeptide derived from human PKC alpha around the phosphorylation site of Thr638

库存 ：999

宿主 ：Rabbit

应用范围 ：WB ELISA IHC-P IHC-F IF

浓度 ：WB 1:500-2000. ELISA 1:500-1000. IHC-P 1:100-500. IHC-F 1:100-500. IF 1:100-500.

靶点 ：Phospho-PRKCA(Thr638)

抗体英文名 ：Anti-Phospho-PRKCA(Thr638) Polyclonal Antibody

抗体名 ：PKC alpha (phospho T638);Phospho-PKC beta II (Thr641);PKC(Phospho-Thr641);Phospho-PKC alpha/beta II (Thr638/641);Phospho-PKC alpha/beta II (Thr638+641)

规格 ：50ul/100ul

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis.