产品详情
文献和实验
相关推荐
注册证号 :咨询销售
英文名 :Primary Human Brain Microvascular Endothelial Cells
CAS号 :咨询销售
库存 :10
供应商 :上海启达生物科技有限公司
肿瘤类型 :咨询销售
细胞类型 :正常细胞
ATCC Number :无
品系 :无
组织来源 :大脑皮层组织
相关疾病 :无
物种来源 :人
免疫类型 :无
细胞形态 :小石子路状
是否是肿瘤细胞 :否
器官来源 :大脑皮层组织
运输方式 :T25瓶/冻管
年限 :5-10年
生长状态 :贴壁生长
Primary Human Brain Microvascular Endothelial Cells,These antibody-free human primary cells were initiated by elutriation of dispase-dissociated normal human brain cortex tissue without the use of positive selection of antibodies.人脑微血管内皮细胞取材于正常人脑皮层组织,dispase-dissociated技术提取纯化,细胞无抗性。
动物种别/Organism:人
组织来源 Tissue and Cell Type:大脑皮层组织
形态/Morphology:小石子路状,贴壁生长
培养基和添加剂/Complete Growth Medium and Culture Conditions:
完全培养基:CultureBoost™经典细胞培养基(货号:4N0-500)
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
冻存液:细胞冻存液Cell Freezing Medium(4Z0-705)
人脑微血管内皮细胞(HBMEC)出库检测指标:
Standard Tests | |
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
Cell Markers and Functional Tests | |
MARKER | RESULTS |
CD31 | > 98% positive by immunofluorescence |
VWF / Factor VIII | > 95% positive by immunofluorescence |
Uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
注意事项:此产品仅供科研使用
cell Systems由著名的细胞生物学家Carl Soderland于1989年在西雅图创立,是分离和制备原代细胞培养物的初创人之一。他们分离出来的原代细胞培养物被制造成“黄金”标准,在大量科学期刊上被引用了数十年。他们的原代细胞使用无抗体分选和培养技术,让实验的结果更为准确,更具有重复性。
参考文献:
Insulin prevents pulmonary vascular leakage by inhibiting transglutaminase 2 in diabetic mice"
Jeon et al. Life Sciences, 2019
"Comprehensive investigation of disease-specific short peptides in sera from patients with systemic sclerosis: Complement C3f-des-arginine, detected predominantly in systemic sclerosis sera, enhances proliferation of vascular endothelial cells" Xiang et al. Arthritis and Rheumatology, 2007.
"Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysis" Furuhata et al.Molecular and Cellular Biochemistry, 2006.
"A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression" Li et al. Journal of Virology, 2000.
上海启达生物科技有限公司
代理商实名认证
钻石会员
入驻年限:5年