保存条件 ：Lyophilized COL G and COL H are stable at -80℃ up to two years. We recommend splitting in aliquots the reconstituted solutions at need and storing them at -20℃ up to one month or -80℃ up to 6 months
保质期 ：Lyophilized COL G and COL H are stable at -80℃ up to two years. We recommend splitting in aliquots the reconstituted solutions at need and storing them at -20℃ up to one month or -80℃ up to 6 months
英文名 ：Recombinant Collagenases Class I (COL G)
库存 ：请询
供应商 ：青木生物技术（武汉）有限公司
CAS号 ：9001-12-1
规格 ：75 U/300 U/750U
1. DESCRIPTION
COL G and COL H are recombinant collagenases (metalloproteinases) class I and class II respectively. COL G and COL H are synthesized separately from C. Histolyticum genes by DNA recombination in E. Coli BL21 AI strain, bearing a Maltose Binding Protein (MBP) tag at the N-terminal end. COL G and COL H are affinity chromatography purified proteins, highly pure, highly stable, lot-tolot consistent, endotoxin-free (≤ 10 EU/mg, LAL assay) and animal-free.
2. APPLICATIONS
Due to their high purity and specificity, COL G and COL H are especially indicated for the isolation of primary cells from liver, pancreas,
heart, cartilage and stem cells from adipose tissue and others. In these applications we recommend using a combination of COL G and COL H in a specific activity ratio, or according to the relevant isolation protocol in order to obtain an optimal collagen digestion in cell isolation. F
该产品被引用文献
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- Optimization of a Biotechnological Process for Production and Purification of Two Recombinant Proteins: Col G and Col H
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