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应用范围 ：Immunoprecipitation;Western Blotting
宿主 ：Rabbit
库存 ：大量
适应物种 ：Rat
抗原来源 ：0
是否单克隆 ：2
规格 ：50 μL
Product Images
Western blotting of rat brain membranes:
1. AB9770, 1:500.
2. AB9770, preincubated with the control peptide antigen.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IH, IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-MaxiK, aa1184-1200.
Alternate Names:
KCA1.1, BKCA, Large conductance Ca2+-activated K+ channel, BKCa, Maxi K+, KCNMA1
Specificity:
Recognizes MaxiK, amino acids 1184-1200 (KCA1.1, BKCA, Large conductance Ca2+-activated K+ channel, BKCa, Maxi K+, KCNMA1).
Key Applications:
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
Western blot: 1:500 using ECL on rat brain membranes.
Immunohistochemistry: On rat brain sections.
Immunoprecipitation: 4 μg using rat brain lysate.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: On rat brain sections.
Immunoprecipitation: 4 μg using rat brain lysate.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Rat
Species Reactivity Note:
The immunogen sequence is identical in human, bovine, chicken, and 16 of 17 amino acids identical in dog.
Immunogen:
Purified peptide from amino acids 1184-1200 of mouse KCa1.1 .
Epitope:
C-terminal intracellular region, STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200.
Control:
Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Gene Symbol:
- KCNMA
- KCNMA1
- SLO1
- KCa1.1
- MAXIK
Antibody Category:
Neuroscience
Antibody Sub-Category:
Ion Channels & Transporters
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Affinity Purified
Host:
Rabbit
Concentration:
0.4 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified peptide from amino acids 1184-1200 of mouse KCa1.1 . 详细描述见链接：http://www.millipore.com/catalogue/item/AB9770-50UL
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