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应用范围 ：Western Blotting;Immunohistochemistry (Paraffin)
宿主 ：Rabbit
库存 ：大量
抗原来源 ：0
适应物种 ：Human;Rat
是否单克隆 ：2
规格 ：500 μL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH(P), WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Ki-67
Background Information:
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Specificity:
Recognizes Ki-67
Key Applications:
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).
Western Blot Analysis:
A previous lot of this antibody was used in western blot.
Optimal working dilutions must be determined by the end user.
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).
Western Blot Analysis:
A previous lot of this antibody was used in western blot.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human and mouse. Expected to react with rat based on sequence homology.
Immunogen:
Synthetic peptide from human Ki-67
Molecular Weight:
Predicted: 359 kDa. MW may be observed at ~345 or 395 kDa
Control:
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.
Presentation:
Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
Gene Symbol:
- MKI67
- KIA
- Ki-67
Quality Assurance:
Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Affinity Purified
Host:
Rabbit
Concentration:
0.2 mg/mL
Antibody Type:
Polyclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Synthetic peptide from human Ki-67. 详细描述见链接：http://www.millipore.com/catalogue/item/AB9260
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入驻年限：9年
