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1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 - 10000 base pairs (bp) by gel electrophoresis. The ladder consists of 13 double-stranded, blunt-end DNA fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1). The 1000 and 3000 bp fragments have greater intensity relative to the other bands for easy identification. DNA Ladder is not intended for quantitative analysis. The 1 kb DNA ladder comes pre-mixed with DNA Loading Dye, which is also available for individual sale (Catalog #79018), for loading DNA samples into agarose gel wells. The recommended loading volume of the 1 kb DNA ladder is 6 μL per lane.STEMCELL Technologies Inc.
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