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保存条件 :2-8 °C
保质期 :12
英文名 :Strep-Tactin® AP conjugate
库存 :999+
供应商 :IBA Lifesciences
规格 :0.5 ml
The streptavidin variant Strep-Tactin® is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin® HRP is applicable for chemiluminescence reaction as well.Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® HRP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.
Specifications
Amount: | 500 µl |
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Concentration: | Strep-Tactin® HRP: 0.95-1.05 mg/ml, Strep-Tactin® AP: 0.175-0.185 mg/ml |
Conjugate: | AP, HRP |
Form: | Suspension in PBS |
Possible Application: | Western blot, detection in microplates |
Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
Shipping information
Storage: | 2-8 °C |
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Stability: | 12 months after shipping |
Shipping: | Room temperature |
IBA Lifesciences
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入驻年限:9年