Strep-Tactin® HRP conjugate

保存条件 ：2-8 °C

保质期 ：12

英文名 ：Strep-Tactin® HRP conjugate

库存 ：999+

供应商 ：IBA Lifesciences

规格 ：0.5 ml

The streptavidin variant Strep-Tactin^® is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag^®II or Twin-Strep-tag^® fusion proteins transferred to a blotting membrane. Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin^® HRP is applicable for chemiluminescence reaction as well.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag^®II or Twin-Strep-tag^® fusion proteins with Strep-Tactin^® HRP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.

Specifications

Amount:	500 µl
Concentration:	Strep-Tactin® HRP: 0.95-1.05 mg/ml, Strep-Tactin® AP: 0.175-0.185 mg/ml
Conjugate:	AP, HRP
Form:	Suspension in PBS
Possible Application:	Western blot, detection in microplates
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage:	2-8 °C
Stability:	12 months after shipping
Shipping:	Room temperature