Strep-Tactin® Superflow® high capacity resin 纯化填料

保存条件 ：2-8°C

保质期 ：6 months

英文名 ：Strep-Tactin® Superflow® high capacity 50% suspension

库存 ：999+

供应商 ：IBA Lifesciences

规格 ：20 ml

Strep-Tactin^® Superflow^® high capacity is a resin for purification of strep-tagged proteins <90 kDa via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of highly cross-linked agarose beads that are coupled to Strep-Tactin^®, which is a streptavidin variant. Strep-Tactin^® binds to proteins that were fused to a Strep-tag^®II or a Twin-Strep-tag^®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin.
In comparison to Strep-Tactin^® Superflow, the high capacity version has a higher density of Strep-Tactin^®, making it suitable for highly expressed proteins. This resin is also an alternative for proteins that are difficult to elute from our Strep-Tactin^®XT 4Flow^® resins.

Please note that biotinylated proteins also bind to Strep-Tactin^® Superflow^® high capacity. If biotinylated proteins are expected, use BioLock.  Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required. 

For further information about our different resins including recommendations, check our resin specifications.

Specifications

Bead Size:	60 - 160 µm spherical
Biotin Binding Capacity:	> 900 nmol/ml resin
Dynamic Protein Binding Capacity:	7 mg/ml resin
Exclusion Limit:	6 x 10^6
Form:	50% suspension
Possible Application:	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support:	6% agarose, crosslinked

Shipping information

Storage:	2-8 °C
Stability:	6 months after shipping
Shipping:	Room temperature