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保存条件 :2-8°C
保质期 :6 months
英文名 :Strep-Tactin® Superflow® resin
库存 :999+
供应商 :IBA Lifesciences
规格 :50 ml
Strep-Tactin® Superflow® is suitable for affinity chromatography-based purification of strep-tagged fusion proteins from cell culture media, cell lysates or other mixed protein preparations. It is optimized for column affinity chromatography and recommended for proteins <90 kDa.The resin consists out of highly cross-linked agarose beads coupled to Strep-Tactin®, a streptavidin variant that binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®. This purification system delivers an outstanding target purity (>95%) without tedious optimization steps due to the specific binding of tagged proteins and a targeted elution. Resin regeneration is possible, enabling multiple purification cycles.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, use BioLock. Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.
If you are unsure if this is the best resin for your approach, have a look at our resin specifications.
Specifications
Bead Size: | 60 - 160 µm spherical |
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Biotin Binding Capacity: | > 300 nmol/ml resin |
Exclusion Limit: | 6 x 10^6 |
Form: | 50% suspension |
Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
Strep-tag®II Binding Capacity: | 3 mg/ml |
Support: | 6% agarose, crosslinked |
Shipping information
Storage: | 2-8 °C |
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Stability: | 6 months after shipping |
Shipping: | Room temperature |
IBA Lifesciences
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入驻年限:9年