Strep-Tactin® Superflow® resin 纯化填料

Strep-Tactin® Superflow® resin 纯化填料

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品牌:IBA Lifesciences 品牌认证

货号:2-1206-025

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保存条件 :2-8°C

保质期 :6 months

英文名 :Strep-Tactin® Superflow® resin

库存 :999+

供应商 :IBA Lifesciences

规格 :50 ml

Strep-Tactin® Superflow® is suitable for affinity chromatography-based purification of strep-tagged fusion proteins from cell culture media, cell lysates or other mixed protein preparations. It is optimized for column affinity chromatography and recommended for proteins <90 kDa.
The resin consists out of highly cross-linked agarose beads coupled to Strep-Tactin®, a streptavidin variant that binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®. This purification system delivers an outstanding target purity (>95%) without tedious optimization steps due to the specific binding of tagged proteins and a targeted elution. Resin regeneration is possible, enabling multiple purification cycles.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, use BioLock.  Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.

If you are unsure if this is the best resin for your approach, have a look at our resin specifications.

Specifications

Bead Size: 60 - 160 µm spherical
Biotin Binding Capacity: > 300 nmol/ml resin
Exclusion Limit: 6 x 10^6
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity: 3 mg/ml
Support: 6% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

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