产品概要

The EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit is designed to isolate CD4+CD127lowCD49d- cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high, CD49d+ cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.

Advantages:
• Fast, easy-to-use and column-free
• Up to 87.4% purity
• Isolated cells are untouched

Components:

• EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
  • EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
  • EasySep™ Dextran Selection Cocktail, 1 mL
  • EasySep™ Magnetic Particles, 3 x 1 mL

• RoboSep™ CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
  • EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
  • EasySep™ Dextran Selection Cocktail, 1 mL
  • EasySep™ Magnetic Particles, 3 x 1 mL
  • RoboSep™ Buffer (Catalog #20104)
  • RoboSep™ Filter Tips (Catalog #20125)

Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)

Cell Type:
T细胞; CD4+T细胞; 调节性T细胞

Species:
人

Sample Source:
PBMC

Selection Method:
负选