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应用范围 ：Chromatin Immunoprecipitation
宿主 ：Mouse
库存 ：大量
抗原来源 ：0
适应物种 ：Human;Mouse;Rat;Yeast (<i>S. cerevisiae</i>)
是否单克隆 ：1
规格 ：200 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Yeast (S. cerevisiae) | Chromatin Immunoprecipitation (ChIP), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-RNA polymerase II, clone CTD4H8
Background Information:
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Clone:
CTD4H8
Specificity:
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
Species Reactivity:
- Human
- Rat
- Mouse
- Yeast (S. cerevisiae)
Species Reactivity Note:
Human, mouse, rat and yeast.
Isotype:
IgG1
Immunogen:
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Molecular Weight:
210-220 kDa
Control:
A431 nuclear extract.
Presentation:
Purified mouse IgG1 in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Quality Assurance:
Routinely evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II 详细描述见链接：http://www.millipore.com/catalogue/item/05-623
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