STEMdiff™脑类器官试剂盒

STEMdiff™ Cerebral Organoid Kit (Catalog #08570) is a defined, serum-free cell culture medium that enables the robust generation of human pluripotent stem cell (hPSC)-derived cerebral organoids in a simple four-stage protocol. Cerebral organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human brain. STEMdiff™ Cerebral Organoid Kit has been optimized to increase efficiency and reproducibility of organoid formation based on the formulation published by Lancaster et al. (Lancaster MA et al. Nature USA, 2013 and Lancaster MA et al. Science USA, 2014). Cerebral organoid formation is initiated through an intermediate embryoid body (EB) formation step followed by expansion of neuroepithelia. After a period of maturation, organoids generated using this kit feature cortical-like regions such as the ventricular zone (PAX6+/SOX2+/Ki-67+), outer subventricular zone (Ki-67+/p-Vimentin+), intermediate zone (TBR2+), and cortical plate (CTIP2+/MAP2+/TBR1+), which layer in similar orientations as observed in vivo. For extended periods of organoid culture (> 40 days), the components required for organoid maturation can be purchased as the STEMdiff™ Cerebral Organoid Maturation Kit (Catalog #08571).

Components:

• STEMdiff™ Cerebral Organoid Kit (Catalog #08570)
  • STEMdiff™ Cerebral Organoid Basal Medium 1,100 mL
  • STEMdiff™ Cerebral Organoid Basal Medium 2, 250 mL
  • STEMdiff™ Cerebral Organoid Supplement A, 10 mL
  • STEMdiff™ Cerebral Organoid Supplement B, 0.5 mL
  • STEMdiff™ Cerebral Organoid Supplement C, 0.25 mL
  • STEMdiff™ Cerebral Organoid Supplement D, 0.5 mL
  • STEMdiff™ Cerebral Organoid Supplement E, 4.5 mL

• STEMdiff™ Cerebral Organoid Maturation Kit (Catalog #08571)
  • STEMdiff™ Cerebral Organoid Basal Medium 2, 250 mL
  • STEMdiff™ Cerebral Organoid Supplement E, 4.5 mL

Cell Type:
PSC衍生神经细胞; 神经干细胞和祖细胞; 多能干细胞

Species:
人

Application:
细胞培养; 分化; 类器官培养

Formulation:
无血清