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提供商 :维百奥生物
服务名称 :SILAC蛋白质组学服务
SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Proteomics ServiceAssay Principle
Stable isotope labeling by amino acids in cell culture (SILAC) is a quantitative proteomic approach. By incorporation of arginine or lysine containing 13C or 15N into the live cell cultures, two groups of cell cultures are distinct. One group of cells grows with "light medium" essentially comprising natural isotope containing amino acids, while another grows with "heavy medium" comprising stable isotope labelled amino acids.
Diagram
Services Included
Cell Culture and Sample Preparation
Labeling incorporation control analysis
SDS-PAGE and Gel fractionation
In-gel digestion and LC-MS/MS analysis
Bioinformatic data analysis
Final report
Sample
Cell: ≥5*10^6 cell
Protein: ≥200ug (≥1ug/ul)
Applications
Study on pathogenesis
Drug target research
Protein modification research
Protein and molecular interaction
Special functional protein screening
维百奥(北京)生物科技有限公司
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入驻年限:6年