dsDNA双链DNA荧光定量检测试剂盒(dsDNA HS Assay Kit for Qubit)价格

保存条件：冰袋运输，2-8℃避光保存6个月。

保质期：6个月

CAS号：无

库存：大量

英文名：Picogreen dsDNA Quantitation Reagent

供应商：翌圣生物科技(上海)股份有限公司

规格：100T(12640ES60)/500T(12640ES76)

qubit试剂搭配酶标仪操作流程

dsDNA HS Assay Kit是一种快速、灵敏、精确的双链DNA（dsDNA）荧光定量检测试剂盒。本试剂盒对dsDNA具有高度选择性，在0.2-100 ng区间具有很好的线性关系。本试剂盒操作简单方便，使用时只需将检测工作液与待测dsDNA样品混合，即可使用Qubit荧光仪或荧光酶标仪进行读数。操作简单，结果可靠，是NGS大规模DNA样品定量（如Input DNA定量、DNA文库定量等）的理想选择。本试剂盒对常规的污染物如蛋白质、盐类等具有较好的耐受性。

产品组分

编号	组分名称	浓度	12640ES60	12640ES76
12640-A	dsDNA Reagent	200×concentrate in DMSO	250 μL	1.25 mL
12640-B	dsDNA Buffer	Not applicable	50 mL	250 mL
12640-C	dsDNA Standard 1	0 ng/μL in TE buffer	1 mL	5×1 mL
12640-D	dsDNA Standard 2	10 ng/μL in TE buffer	1 mL	5×1 mL

运输和保存
冰袋运输，2-8℃避光保存12个月。

注意事项
1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭；
2）对于DNA标准品，每次使用前先上下颠倒数次后瞬时离心数秒（请勿涡旋振荡）；
3）为了您的安全和健康，请穿实验服并戴一次性手套操作。
4）本产品仅用作科研用途！

实验步骤
一、使用Qubit荧光仪进行dsDNA定量检测分析

污染物对dsDNA定量检测试剂盒检测结果的影响

污染物	10 µL样品中的浓度	样品检测时浓度	检测结果
Salts
Ammonium acetate	200 mM	10 mM	OK
Sodium acetate	200 mM	10 mM	OK
Sodium chloride	200 mM	10 mM	OK
Magnesium chloride	40 mM	2 mM	OK
Organic Solvents
Phenol	2%	0.1%	OK
Ethanol	20%	1%	OK
Chloroform	4%	0.2%	OK
Detergents
Sodium dodecyl sulfate	0.2%	0.01%	OK
Triton X-100	0.02%	0.001%	OK
Other Compounds
Bovine serum albumin	400 μg/mL	20 μg/mL	OK
RNA	1×*	1×*	OK
dNTPs	2 mM	100 μM	OK
Polyethylene glycol	20%	1%	OK
Agarose	2%	0.1%	OK

*1×：表示含有与dsDNA相同浓度的RNA。

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文库定量	产品编号	规格
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HB221117

该产品被引用文献

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