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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum, plasma(EDTA) and urine.
规格 ：96T
产品概况
| 货号 | EK1103-CN |
|---|---|
| 产品名称 | Dog Canine TGF-Beta 3 ELISA Kit |
| 产品描述 | Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Dog Caninea TGF-beta 3. 96wells/kit, with removable strips. |
| 免疫原 | Expression system for standard: NS0; Immunogen sequence: A346-S457 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | TGFB3 |
| 基因全名 | Transforming growth factor beta-3 |
| Uniprot ID | TGFB3: A0A8C0SQX7/A0A8I3S1P1 |
| 指标别名 | Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;TGFB3; |
| 背景资料 | Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 | 10ng/tube
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| n="3">I | ||||||
| ntra-Assay Precisio | ||||||
| n | ||||||
| n="3">I | ||||||
| nter-Assay Precisio | ||||||
| n | ||||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 271 | 631 | 1153 | 294 | 670 | 1265 |
| Standard deviation | 14.4 | 29 | 45 | 18.82 | 36.4 | 72.85 |
| CV(%) | 5.3 | 4.6 | 3.9 | 6.4 | 5.7 | 5.8 |
Dog Canine TGF-Beta 3 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 |
| O.D. | 0.035 | 0.121 | 0.220 | 0.391 | 0.671 | 1.043 | 1.608 | 2.051 |
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