产品详情
文献和实验
相关推荐
库存 :发货周期:现货
供应商 :博士德生物
应用 :cell culture supernates, serum and plasma (heparin, EDTA, citrate).
样本 :Rat
规格 :96T
产品概况
货号 | EK1742 |
---|---|
产品名称 | Rat IL6ST ELISA Kit |
产品描述 | For quantitative detection of rat IL6ST in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, Q23-E618 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.05ng/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | Il6st |
基因全名 | interleukin 6 signal transducer |
Uniprot ID | P40190 |
指标别名 | Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st |
背景资料 | GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1742-CAP | 96-well plate precoated with anti- rat IL6ST antibody | 1 |
EK1742-ST | Lyophilized recombinant rat IL6ST standard | 50ng/tube×2 |
EK1742-DA | Biotinylated anti- rat IL6ST antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1. 96孔读板仪器。
2. 自动洗板机.
3. 移液器和枪头。建议使用多通道移液器/排枪。
4. Eppendorf 管。
5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1254 | 4579 | 20188 | 1322 | 5546 | 21456 |
Standard deviation | 66.46 | 290.3 | 847.9 | 99.15 | 321.7 | 1652.1 |
CV(%) | 5.3% | 6.1% | 4.2% | 7.5% | 5.8% | 7.7% |
Rat IL6ST ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.008 | 0.329 | 0.516 | 0.817 | 1.102 | 1.336 | 1.568 | 1.839 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限:17年