Mouse Rat Triiodothyronine (T3) Total ELISA Kit

库存 ：发货周期：4-8周

供应商 ：博士德生物

应用 ：Serum

样本 ：Mouse, Rat

规格 ：96T

Introduction

This ELISA kit is of competitive format. Competitive ELISA, also known as inhibition ELISA, is a surface/plate based assay, where the plate is coated with capture antibodies reactive to the molecule of interest. The sample (containing native molecule of interest) and enzyme conjugated recombinant protein (the competing molecule) are added to the coated wells. Since the amount of enzyme conjugated molecule in each well is constant, the level of native molecule in the sample will determine the binding ratio of enzyme conjugated molecule vs. native molecule. After an incubation period, any unbound antibody is washed off. Enzyme substrate (for example, TMB for HRP) is added to each well and will be transformed into a blue precipitate, the amount of which is linearly proportional to the amount of enzyme in the well. The precipitate is then turned into yellow by adding the acid stop solution and the concentration of yellow precipitate is read at 450nm for light absorbance (O.D. value). The O.D. is then used to calculate the amount of molecule of interest in each well, by comparing each sample well against the standard curve. The standard curve is generated using the same principle but instead of adding samples, a series of recombinant molecules with known concentrations are added to 6-8 wells.

Overview

Product Name	Mouse Rat Triiodothyronine (T3) Total ELISA Kit
SKU/Catalog Number	EK7011
Description	Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Triiodothyronine (T3). 96wells/kit, with removable strips.
Cite This Product	Mouse Rat Triiodothyronine (T3) Total ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7011)
Validated Species	Mouse, Rat
Application	ELISA *Our Boster Guarantee covers the use of this product in the above tested applications.
Cross Reactivity	There is no detectable cross-reactivity.
Pack Size	96wells/kit, with removable strips.

Properties

Sensitivity	0.25 ng/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range	0.25-7.5 ng/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type	Serum *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.  **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage	Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Description	Quantity
Microwells coated with T3 Monoclonal Ab	12x8x1 Microwells
T3 Standards	7 vials ( ready to use) 0.25 ml
T3 Enzyme Conjugate concentrate	1 vial 1.5ml
Assay Diluent	1 bottle (ready to use) 12ml
TMB Substrate	1 bottle (ready to use) 12ml
Stop Solution	1 bottle (ready to use) 12ml
20X Wash concentrate	1 bottle 25ml

Material Required But Not Provided

1. Distilled or deionized water

2. Precision pipettes

3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.

4. ELISA reader capable of reading absorbance at 450nm

5. Absorbance paper or paper towel

6. Graph paper

Background

Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Production of T3 and its prohormone thyroxine (T4) is activated by thyroid-stimulating hormone (TSH), which is released from the anterior pituitary gland.

Mouse Rat Triiodothyronine (T3) Total ELISA Kit Images

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