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库存 :现货
供应商 :博士德生物
检测限 :31.2pg/ml-2000pg/ml
检测方法 :酶联免疫双抗夹心法
应用 :cell culture supernates, serum and plasma (heparin, EDTA).
适应物种 :Mouse
规格 :96T
产品概况
货号 | EK1894 |
---|---|
产品名称 | Mouse HTRA2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse HTRA2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, A134-E458 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Htra2 |
基因全名 | HtrA serine peptidase 2 |
Uniprot ID | Q9JIY5 |
指标别名 | Serine protease HTRA2, mitochondrial; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI; HTRA2; OMI; PRSS25 |
背景资料 | Serine protease HTRA2, mitochondrial is an enzyme that in humans is encoded by the HTRA2 gene. It is mapped to 6 C3; 6 35.94 cM. This protein is involved in caspase-dependent apoptosis and in Parkinson's disease. Protein HtrA2, also known as Omi, is a mitochondrially-located serine protease. The human protein Serine protease HTRA2, mitochondrial is 49kDa in size and composed of 458 amino acids. The peptide fragment of 1-31 amino acid is the mitochondrial transition sequence, fragment 32-133 amino acid is propertied, and 134-458 is the mature protein Serine protease HTRA2, mitochondrial, and its theoretical pI of this protein is 6.12. HtrA2 shows similarities with DegS, a bacterial protease present in the periplasm of gram-negative bacteria. Structurally, HtrA2 is a trimeric molecule with central protease domains and a carboxy-terminal PDZ domain, which is characteristic of the HtrA family. The PDZ domain preferentially binds C-terminus of the protein substrate and modulate the proteolytic activity of the trypsin-like protease domain. |
显色时间 | 15-20 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1894-CAP | 96-well plate precoated with anti- mouse HTRA2 antibody | 1 |
EK1894-ST | Lyophilized recombinant mouse HTRA2 standard | 10ng/tube×2 |
EK1894-DA | Biotinylated anti- mouse HTRA2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 95 | 243 | 1003 | 99 | 247 | 1109 |
Standard deviation | 6.37 | 17.98 | 50.15 | 6.14 | 11.61 | 86.50 |
CV(%) | 6.7% | 7.4% | 5.0% | 6.2% | 4.7% | 7.8% |
Mouse HTRA2 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20)Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.005 | 0.105 | 0.237 | 0.338 | 0.712 | 1.124 | 1.876 | 2.211 |
武汉博士德生物工程有限公司
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入驻年限:17年