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库存 :发货周期:现货
供应商 :博士德生物
应用 :cell culture supernates, serum and plasma (heparin, EDTA).
样本 :Mouse
规格 :96T
产品概况
货号 | EK1854 |
---|---|
产品名称 | Mouse PDGF-CC ELISA Kit |
产品描述 | For quantitative detection of mouse PDGF-CC in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, V235-G345 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Pdgfc |
基因全名 | platelet-derived growth factor, C polypeptide |
Uniprot ID | Q8CI19 |
指标别名 | Platelet-derived growth factor C; PDGF-C; Fallotein; Spinal cord-derived growth factor; SCDGF; VEGF-E; Platelet-derived growth factor C, latent form; PDGFC latent form; Platelet-derived growth factor C, receptor-binding form; PDGFC receptor-binding form; Pdgfc; Scdgf |
背景资料 | Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. It is mapped to 3; 3 E3. Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation. |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1854-CAP | 96-well plate precoated with anti- mouse PDGF-CC antibody | 1 |
EK1854-ST | Lyophilized recombinant mouse PDGF-CC standard | 10ng/tube×2 |
EK1854-DA | Biotinylated anti- mouse PDGF-CC antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 20 | 104 | 310 | 24 | 116 | 425 |
Standard deviation | 1.18 | 4.47 | 12.71 | 1.58 | 6.73 | 28.05 |
CV(%) | 5.9% | 4.3% | 4.1% | 6.6% | 5.8% | 6.6% |
Mouse PDGF-CC ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.231 |
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入驻年限:17年