Human CA-125 ELISA Kit

库存 ：发货周期:4-8周

供应商 ：博士德生物

应用 ：Serum

规格 ：96T

Introduction

The Bosterbio OneStep Human CA125 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CA125 with a 96-well strip plate that is pre-coated with antibody specific for CA125. The detection antibody is a HRP conjugated antibody specific for CA125. The capture antibody is monoclonal antibody from mouse, and the detection antibody is monoclonal antibody from mouse. The kit is analytically validated with ready to use reagents. To measure Human CA125, add standards and samples to the wells, then add the HRP conjugated detection antibody. Wash away the unbounded protein and HRP conjugated detection antibody. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. Upon addition of the substrate, the density of the yellow product is linearly propotional to Human CA125 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CA125 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster's ELISA Resource Center at https://www.bosterbio.com/elisatechnical-resource-center.

Overview

Product Name	Human CA-125 ELISA Kit
SKU/Catalog Number	EK7029
Description	Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA125. 96wells/kit, with removable strips.
Cite This Product	Human CA-125 OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7029)
Validated Species	Human
Application	ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
Cross Reactivity	There is no detectable cross-reactivity.
Pack Size	96wells/kit, with removable strips.

Properties

Sensitivity	3.8 U/ml  *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range	15-300 U/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type	Serum *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.  **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage	Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human CA125 Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti- CA125 monoclonal antibody, Polystyrene micro-well plate
Human CA125 Standards(S1~S4)	4	0.5ml	CA125 (from natural protein) (15，41，120, 300) U/ml, 0.02M PBS, 20% new-born calf serum
HRP Conjugated anti-Human CA125 antibody	1	6ml	HRP Conjugated anti-Human CA125 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody
Controls	1	0.5ml	CA125 (from natural protein), 0.02M PBS, 20% new-born calf serum
20X Wash Buffer Concentrate	1	15ml	Neutral buffer, contains 0.5% Tween-20
Color Developing Reagent A	1	7ml	Contains 11mmol/L H2O2
Color Developing Reagent B	1	7ml	Contains 2mmol/L TMB
Stop Solution	1	7ml	2mol/L dilute sulphuric acid
Plate Sealers	2	Piece

Material Required But Not Provided

1. Microplate Reader capable of reading absorbance at 450nm.

2. Automated plate washer (optional)

3. Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples.

4. Deionized or distilledwater.

5. 500ml graduated cylinders.

6. Test tubes for dilution.

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name	MUC16
Uniprot ID	Q8WXI7
Reseach Areas	Cancer, Cell Type Markers, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens|

*if product is indicated to react with multiple species, protein info is based on the human gene.

Human CA-125 OneStep ELISA Kit Images

Click the images to enlarge.

[list_product_images] |Human CA125 ELISA Kit standard curve [/list_product_images]