Human High Sensitivity C-Reactive Protein (CRP) ELISA Kit

库存 ：发货周期:4-8周

供应商 ：博士德生物

应用 ：SerumandPlasma

规格 ：96T

Introduction

The Bosterbio OneStep ELISA kit is a solid phase direct ELISA sandwich kit. Instead of adding samples detection antibody and ABC-HRP separately, The OneStep ELISA kit allows the user to add standards, samples and controls to wells in one step, along with the incubation buffer. After a simple washing step, an enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.

Overview

Product Name	Human High Sensitivity C-Reactive Protein (CRP) ELISA Kit
SKU/Catalog Number	EK7040
Description	Human High Sensitivity C-Reactive Protein (CRP) ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.
Cite This Product	Human High Sensitivity C-Reactive Protein (CRP) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7040)
Validated Species	Human
Application	ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
Cross Reactivity	There is no detectable cross-reactivity.
Pack Size	96wells/kit, with removable strips.

Properties

Sensitivity	0.005 mg/L *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range	0.005-0.1 mg/L *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type	Serum and Plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.  **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage	Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Description	Quantity
1. Microwells coated with CRP MAb	12x8x1
2. CRP Standard: 6 vials (ready to use)	0.7ml
3. CRP Enzyme Conjugate: 1 bottle (ready to use)	12 ml
4. TMB Substrate: 1 bottle (ready to use)	12ml
5. Stop Solution: 1 bottle (ready to use)	12ml
6. Sample Diluent	50 ml
7. 20X Wash concentrate: 1 bottle	25ml

Material Required But Not Provided

1. Distilled or deionized water

2. Precision pipettes

3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.

4. ELISA reader capable of reading absorbance at 450nm

5. Absorbance paper or paper towel

6. Graph paper

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name	NR3C1
Uniprot ID	P04150

*if product is indicated to react with multiple species, protein info is based on the human gene.

Human High Sensitivity C-Reactive Protein (CRP) ELISA Kit Images

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