Human Scl-70 Autoantibody IgG ELISA Kit (Direct EIA)

库存 ：发货周期:4-8周

供应商 ：博士德生物

应用 ：SerumandPlasma

规格 ：96T

Introduction

Diluted patient serum is added to wells coated with purified antigens. If antibody against target antigen is present, the antibody will bind to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of the color developed is proportional to the amount of IgG specific antibody in the sample.

Overview

Product Name	Human Scl-70 Autoantibody IgG ELISA Kit (Direct EIA)
SKU/Catalog Number	EK7087
Description	Human Scl-70 Autoantibody IgG ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.
Cite This Product	Human Scl-70 Autoantibody IgG ELISA Kit (Direct EIA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7087)
Validated Species	Human
Application	ELISA *Our Boster Guarantee covers the use of this product in the above tested applications.
Cross Reactivity	There is no detectable cross-reactivity.
Pack Size	96wells/kit, with removable strips.

Properties

Sample Type	Serum and Plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.  **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage	Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Kit Components

Description	Quantity
1. Microwells coated with ScI-70 antigen	12x8x1
2. Sample Diluent: 1 bottle (ready to use)	22 ml
3. Calibrator: 1 Vial (ready to use)	1ml
4. Positive Control: 1 vial (ready to use)	1ml
5. Negative Control: 1 vial (ready to use)	1ml
6. Enzyme conjugate: 1 bottle (ready to use)	12ml
7. TMB Substrate: 1 bottle (ready to use)	12ml
8. Stop Solution: 1 bottle (ready to use)	12ml
9. Wash concentrate 20X: 1 bottle	25ml

Material Required But Not Provided

1. Distilled or deionized water

2. Precision pipettes

3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.

4. ELISA reader capable of reading absorbance at 450nm

5. Absorbance paper or paper towel

6. Graph paper

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