产品详情
文献和实验
相关推荐
库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma (heparin, EDTA).
规格 ：96T
产品概况
| 货号 | EK1743 |
|---|---|
| 产品名称 | Human VWF-A2 ELISA Kit |
| 产品描述 | For quantitative detection of human VWF-A2 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, D1498-V1665 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | VWF |
| 基因全名 | von Willebrand factor |
| Uniprot ID | P04275 |
| 指标别名 | von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF |
| 背景资料 | Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of plateletvessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1743-CAP | 96-well plate precoated with anti- human VWF-A2 antibody | 1 |
| EK1743-ST | Lyophilized recombinant human VWF-A2 standard | 20ng/tube×2 |
| EK1743-DA | Biotinylated anti- human VWF-A2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 571 | 1728 | 9762 | 600 | 1987 | 9834 |
| Standard deviation | 35.4 | 73.3 | 497.86 | 40.2 | 147.04 | 717.9 |
| CV(%) | 6.2% | 4.3% | 5.1% | 6.7% | 7.4% | 7.3% |
Human VWF-A2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.037 | 0.180 | 0.306 | 0.511 | 1.013 | 1.448 | 2.089 | 2.531 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
