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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma (heparin, EDTA).
规格 ：96T
产品概况
| 货号 | EK1946 |
|---|---|
| 产品名称 | Human Neudesin ELISA Kit |
| 产品描述 | For quantitative detection of human Neudesin in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, G32-F172 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 93.6pg/ml-6000pg/ml |
| 基因名称 | NENF |
| 基因全名 | neudesin neurotrophic factor |
| Uniprot ID | Q9UMX5 |
| 指标别名 | Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF |
| 背景资料 | This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. It is mapped to 1q32.3. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1946-CAP | 96-well plate precoated with anti- human Neudesin antibody | 1 |
| EK1946-ST | Lyophilized recombinant human Neudesin standard | 10ng/tube×2 |
| EK1946-DA | Biotinylated anti- human Neudesin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 124 | 715 | 2078 | 159 | 739 | 2238 |
| Standard deviation | 7.69 | 32.18 | 118.45 | 7.79 | 42.86 | 140.99 |
| CV(%) | 6.2% | 4.5% | 5.7% | 4.9% | 5.8% | 6.3% |
Human Neudesin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 93.6 | 187 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.058 | 0.119 | 0.181 | 0.257 | 0.482 | 0.910 | 1.522 | 2.263 |
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