产品详情
文献和实验
相关推荐
库存 ：发货周期:7-8周
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma (heparin, EDTA, citrate).
规格 ：96T
产品概况
| 货号 | EK2069 |
|---|---|
| 产品名称 | Human GFRA1 ELISA Kit |
| 产品描述 | For quantitative detection of human GFRA1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, D25-S429 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 46.9pg/ml-3000pg/ml |
| 基因名称 | GFRA1 |
| 基因全名 | GDNF family receptor alpha 1 |
| Uniprot ID | GFRA1: P56159 |
| 指标别名 | GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; GFRA1; GDNFRA; RETL1; TRNR1 |
| 背景资料 | GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 10q25.3. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2069-CAP | 96-well plate precoated with anti- human GFRA1 antibody | 1 |
| EK2069-ST | Lyophilized recombinant human GFRA1 standard | 10ng/tube×2 |
| EK2069-DA | Biotinylated anti- human GFRA1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 67 | 235 | 1418 | 74 | 261 | 1425 |
| Standard deviation | 3.95 | 10.11 | 58.14 | 4.88 | 15.14 | 94.05 |
| CV(%) | 5.9% | 4.3% | 4.1% | 6.6% | 5.8% | 6.6% |
Human GFRA1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(ng/ml) | 0 | 46.9 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.039 | 0.130 | 0.242 | 0.507 | 0.824 | 1.311 | 1.776 | 2.194 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
