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克隆性 ：Polyclonal
适应物种 ：human, mouse, rat
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, IHC, IHC-F, ICC/IF, FCM
规格 ：50μl/100μl/150μl
产品概况
| 货号 | A00761-1 |
|---|---|
| 产品名称 | Anti-PAH Antibody |
| 基因名 | PAH |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 52KD |
| 免疫原 | E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. |
| 研究类别 | 1. Fitzpatrick PF (1999). "Tetrahydropterin-dependent amino acid hydroxylases". Annual Review of Biochemistry. 68: 355–81.2. Kaufman S (Feb 1958). "A new cofactor required for the enzymatic conversion of phenylalanine to tyrosine". The Journal of Biological Chemistry. 230 (2): 931–9. |
| Uniprot ID | PAH: P00439 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PAH antibody (A00761-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human liver tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
Use rabbit anti- PAH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for PAH at approximately 52KD. The expected band size for PAH is at 52KD.|Figure 2. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of human renal cancer tissue. anti-PAH Antibody (A00761-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of mouse kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of rat kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IF analysis of PAH using anti- PAH antibody (A00761-1).
PAH was detected in immunocytochemical section of HepG2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 6. Flow Cytometry analysis of HepG2 cells using anti- PAH antibody (A00761-1).
Overlay histogram showing HepG2 cells stained with A00761-1 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human liver tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
Use rabbit anti- PAH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for PAH at approximately 52KD. The expected band size for PAH is at 52KD.|Figure 2. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of human renal cancer tissue. anti-PAH Antibody (A00761-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of mouse kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of PAH using anti-PAH antibody (A00761-1).PAH was detected in paraffin-embedded section of rat kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IF analysis of PAH using anti- PAH antibody (A00761-1).
PAH was detected in immunocytochemical section of HepG2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 6. Flow Cytometry analysis of HepG2 cells using anti- PAH antibody (A00761-1).
Overlay histogram showing HepG2 cells stained with A00761-1 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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