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克隆性 :Polyclonal
适应物种 :human
保质期 :发货周期:现货
库存 :9999
供应商 :博士德生物
应用范围 :WB
规格 :50μl/100μl/150μl
产品概况
货号 | A04946 |
---|---|
产品名称 | Anti-PREB Antibody |
基因名 | PREB |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 45KD |
免疫原 | E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB. |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene. This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. |
研究类别 | 1. Edgar AJ (Jul 2003). "The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12". BMC Genomics. 4: 18.2. Fliss MS, Hinkle PM, Bancroft C (May 1999). "Expression cloning and characterization of PREB (prolactin regulatory element binding), a novel WD motif DNA-binding protein with a capacity to regulate prolactin promoter activity". MolEndocrinol. 13 (4): 644–657. |
Uniprot ID | PREB: Q9HCU5 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of PREB using anti-PREB antibody (A04946). Lane 1: human Hela whole cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human A549 whole cell lysate. anti-PREB antigen affinity purified polyclonal antibody (Catalog # A04946)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for PREB at approximately 45KD. The expected band size for PREB is at 45KD.[/list_product_images]
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