Anti-IL-1 beta Antibody价格_品牌:BOSTER-丁香通官网

克隆性：Polyclonal

适应物种：mouse

保质期：发货周期：现货

库存：9999

供应商：博士德生物

应用范围：WB, ELISA

规格：50μl/100μl/150μl

产品概况

货号	A00101-1
产品名称	Anti-IL-1 beta Antibody
基因名	Il1b
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	36KD
免疫原	E.coli-derived mouse IL-1 beta/Il1b recombinant protein (Position: V118-S269).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
研究类别	1. Baek, S. H.; Ohgi, K. A.; Rose, D. W.; Koo, E. H.; Glass, C. K.; Rosenfeld, M. G. : Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110: 55-67, 2002.2. Chen, C.-N.; Li, Y.-S. J.; Yeh, Y.-T.; Lee, P.-L.; Usami, S.; Chien, S.; Chiu, J.-J. : Synergistic roles of platelet-derived growth factor-BB and interleukin-1-beta in phenotypic modulation of human aortic smooth muscle cells. Proc. Nat. Acad. Sci. 103: 2665-2670, 2006. 3. Takahashi, J. L.; Giuliani, F.; Power, C.; Imai, Y.; Yong, V. W. : Interleukin-1-beta promotes oligodendrocyte death through glutamate excitotoxicity. Ann. Neurol. 53: 588-595, 2003. 4. Voronov, E.; Shouval, D. S.; Krelin, Y.; Cagnano, E.; Benharroch, D.; Iwakura, Y.; Dinarello, C. A.; Apte, R. N. : IL-1 is required for tumor invasiveness and angiogenesis. Proc. Nat. Acad. Sci. 100: 2645-2650, 2003.
Uniprot ID	Il1b: P10749
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:500-2000
ELISA：	1:100-1000

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- IL1 beta antibody (A00101-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse RAW264.7(-LPS) whole cell lysates,
Lane 2: mouse RAW264.7(+LPS) whole cell lysates.
Use rabbit anti- IL1 beta 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IL1 beta at approximately 36KD. The expected band size for IL1 beta is at 31KD.[/list_product_images]