Anti-Flag Tag Antibody

价格： ￥680 - 1180

品牌：BOSTER 品牌认证

货号：M30971

产品详情

产品概况

货号	M30971
产品名称	Anti-Flag Tag Antibody
抗体来源	Mouse
克隆	Monoclonal(Clone:1E6)
抗体亚型	Mouse
分子量	45KD
免疫原	This antibody is produced by immunizing animals with a synthetic Flag tag (DYKDDDDK) peptide corresponding to KLH.
内容	500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.
浓度	0.5mg/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot（WB）:	1:500-5000

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Western blot analysis of different concentration of Anti-Flag Tag(1E6) Monoclonal Antibody (M30971)
Blocking: Incubate the membrane with 5% Non-Fat Milk, 1×PBS, 4℃, overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1×TBS, 0.1% Tween-20, RT, 30mins[/list_product_images]