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克隆性 :Polyclonal
适应物种 :human
保质期 :发货周期:现货
库存 :9999
供应商 :博士德生物
应用范围 :WB, IHC, IHC-F, ICC/IF, FCM, ELISA
规格 :50μl/100μl/150μl
产品概况
货号 | A03628-1 |
---|---|
产品名称 | Anti-LMAN1 Antibody |
基因名 | LMAN1 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 58KD |
免疫原 | E. coli-derived human LMAN1 recombinant protein (Position: K96-M458). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Protein ERGIC-53 also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1 is a protein that in humans is encoded by the LMAN1 gene. It is mapped to 18q21.32. The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. |
研究类别 | 1. Neve, E. P. A., Svensson, K., Fuxe, J., Petterson, R. F. VIPL, a VIP36-like membrane protein with a putative function in the export of glycoproteins from the endoplasmic reticulum. Exp. Cell Res. 288: 70-83, 2003. 2. Zhang, B., Spreafico, M., Zheng, C., Yang, A., Platzer, P., Callaghan, M. U., Avci, Z., Ozbek, N., Mahlangu, J., Haw, T., Kaufman, R. J., Marchant, K., Tuddenham, E. G. D., Seligsohn, U., Peyvandi, F., Ginsburg, D. Genotype-phenotype correlation in combined deficiency of factor V and factor VIII. Blood 111: 5592-5600, 2008. |
Uniprot ID | LMAN1: P49257 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Immunohistochemistry in frozen section: | 1:50-400 |
Immunocytochemistry in fixed cells: | 1:50-400 |
Flow cytometry (FCM): | 1-3 μg/1x106 cells |
(ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysate,Lane 2: human A431 whole cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for LMAN1 at approximately 58KD. The expected band size for LMAN1 is at 58KD.|Figure 2. IHC analysis of LMAN1 using anti-LMAN1 antibody (A03628-1).LMAN1 was detected in paraffin-embedded section of human appendicitis tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of LMAN1 using anti-LMAN1 antibody (A03628-1).LMAN1 was detected in paraffin-embedded section of human glioma tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of LMAN1 using anti-LMAN1 antibody (A03628-1).LMAN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of LMAN1 using anti-LMAN1 antibody (A03628-1).LMAN1 was detected in paraffin-embedded section of human placenta tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of LMAN1 using anti-LMAN1 antibody (A03628-1).LMAN1 was detected in paraffin-embedded section of human tonsil tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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