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免疫原 :A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)
亚型 :Rabbit IgG
形态 :Liquid
保存条件 :12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
克隆性 :单克隆
适应物种 :Human,Mouse,Rat
抗原来源 :Rabbit
库存 :5-7天
供应商 :博士德生物
应用范围 :WB
浓度 :0
规格 :50ul/100ul/150ul
产品概况
货号 | BM5446 |
---|---|
产品名称 | Anti-Phospho-ERK1 (T202) + ERK2 (T185) Antibody |
抗体来源 | Rabbit |
克隆 | Monoclonal (Clone: ) |
抗体亚型 | Rabbit IgG |
免疫原 | A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185) |
内容 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. |
Uniprot ID | : P27361 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot: | 1:100-400 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Western blot analysis of Phospho-ERK1 (T202) + ERK2 (T185) expression in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.[/list_product_images]
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入驻年限:17年