Anit-SCARB1 Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human,mouse,rat

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：IF,WB

浓度 ：0

规格 ：200ul

产品概况

产品名称	Anti-SCARB1 Antibody
产品描述	Anti-SCARB1 Rabbit Monoclonal Antibodytested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse, Rat
文献引用格式	Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # BM4233)
应用范围	IF, WB
抗体来源	Rabbit
抗体亚型	Rabbit IgG
推荐配套的二抗和检测试剂	Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(F) and ICC.
浓度	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
储存条件	At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	SCARB1
基因全名	scavenger receptor class B, member 1
Uniprot ID	SCARB1: Q8WTV0

指标背景简介

Scavenger receptor class B member 1 (SRB1), also known as SR-BI, is a protein that in humans is encoded by the SCARB1 gene. SR-BI functions as a receptor for high-density lipoprotein. Scavenger receptor class B, type I (SR-BI) is an integral membrane protein found in numerous cell types/tissues, including the liver and adrenal. It is best known for its role in facilitating the uptake of cholesteryl esters from high-density lipoproteins in the liver. This process drives the movement of cholesterol from peripheral tissues towards the liver for excretion. This movement of cholesterol is known as reverse cholesterol transport and is a protective mechanism against the development of atherosclerosis, which is the principal cause of heart disease and stroke. SR-BI has also been identified in the livers of non-mammalian species (turtle, goldfish, shark, chicken, frog, and skate), suggesting it emerged early in vertebrate evolutionary history. The turtle also seems to upregulate SB-RI during egg development, indicating that cholesterol efflux may be at peak levels during developmental stages.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot	1:500-1:2000
Immunohistochemistry(Frozen Section)	1:50-1:200

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]Western blot analysis of SCARB1 expression in (1) Human fetal liver lysate; (2) Mouse liver lysate (BM4233). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARB1 monoclonal antibody (Catalog # BM4233) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARB1.[/list_product_images]